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Molecular Biology and Genetics Lab

Molecular Biology and Genetics Lab

Molecular Biology and Genetic lab’s instrumentation

Molecular Biology and Genetic labarotory is equipped with the most modern intruments that allow students and researchers to do advanced work in their particular areas. Correct sample throughput and accuracy are the most important two factors for a labarotory working at molecular level.

 

Molecular Biology and Genetic lab’s experiments include:

Ø Gene cloning

Ø Gene transfer

Ø Protein detecting techniques

  • Protein immunostaining
  • Protein immunoprecipitation
  • Immunoelectrophoresis
  • Immunoblotting
  • Western blot
  • Spectrophotometry
  • Enzyme assay
  • SDS-PAGE Electrophoresis
  • Chemiluminescent detection system for protein detection
  • 2D Gel Electrophoresis

Ø Protein purification

Ø Protein Isolation

Ø Chromatography methods

  • Ion exchange
  • Size-exclusion chromatography or Gelfiltration
  • Affinity chromatography

Ø Protein Determination

Ø Radioactive isotope labeling

Ø DNA and RNA Purification

Ø Quantification of DNA and RNA (PCR and qPCR)

Ø Agarose Gel electrophoresis

Ø DNA sequencing

Ø Expression cloning

Ø Southern blot

Ø Northern blot

Ø Radioactivity in biological research